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differentiation 44 cd44  (Miltenyi Biotec)


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    Miltenyi Biotec differentiation 44 cd44
    Differentiation 44 Cd44, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/differentiation+44+cd44/CD44+Antibody%2C+anti-human%2C+REAfinity/pm41083750-295-17-26
    Average 95 stars, based on 80 article reviews
    differentiation 44 cd44 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: In-depth analysis of obesity-associated changes in adipose tissue-derived mesenchymal stromal/stem cells and primary cilia function
    Article Snippet: Cells were trypsinized with 0.25% trypsin (Sigma-Aldrich), harvested, and fixed for 15 min with ice-cold 2% paraformaldehyde. .. After washing twice with PBS, the cells were stained with the following antibodies: PE-conjugated anti-human cluster of differentiation 44 (CD44) (#130-113-342, clone REA690, lot 5240507442, MACS Miltenyi Biotec, Bergisch Gladbach), PE-conjugated anti-human CD73 (#550257, lot 4149735, BD-Pharmingen, Heidelberg), FITC-CD90 (#11-0909-42, clone eBio5E10, lot 2648893, eBioscience, Frankfurt), PE-conjugated anti-human CD105 (#323206, clone 43A3, lot B418585, BioLegend, San Diego), PerCP-Cy5.5-conjugated anti-human CD14 (#45-0149-42, clone 61D3, lot 2785933, eBioscience), APC-conjugated anti-human CD31 (#17-0319-42, clone WM-59, lot 2702043, eBioscience), and FITC-conjugated anti-human CD34 (#343504, clone 581, lot B407007, eBioscience). ..

    Article Title: In-depth analysis of obesity-associated changes in adipose tissue-derived mesenchymal stromal/stem cells and primary cilia function.
    Article Snippet: Cells were trypsinized with 0.25% trypsin (Sigma-Aldrich), harvested, and fixed for 15min with icecold 2% paraformaldehyde. .. After washing twice with PBS, the cells were stained with the following antibodies: PE-conjugated anti-human cluster of differentiation 44 (CD44) (#130-113-342, clone REA690, lot 5240507442, MACS Miltenyi Biotec, Bergisch Gladbach), PE-conjugated anti-human CD73 (#550257, lot 4149735, BD-Pharmingen, Heidelberg), FITC-CD90 (#11-0909-42, clone eBio5E10, lot 2648893, eBioscience, Frankfurt), PEconjugated anti-human CD105 (#323206, clone 43A3, lot B418585, BioLegend, San Diego), PerCP-Cy5.5-conjugated anti-human CD14 (#45- 0149-42, clone 61D3, lot 2785933, eBioscience), APC-conjugated antihuman CD31 (#17-0319-42, clone WM-59, lot 2702043, eBioscience), and FITC-conjugated anti-human CD34 (#343504, clone 581, lot B407007, eBioscience). ..

    Magnetic Cell Separation:

    Article Title: In-depth analysis of obesity-associated changes in adipose tissue-derived mesenchymal stromal/stem cells and primary cilia function
    Article Snippet: Cells were trypsinized with 0.25% trypsin (Sigma-Aldrich), harvested, and fixed for 15 min with ice-cold 2% paraformaldehyde. .. After washing twice with PBS, the cells were stained with the following antibodies: PE-conjugated anti-human cluster of differentiation 44 (CD44) (#130-113-342, clone REA690, lot 5240507442, MACS Miltenyi Biotec, Bergisch Gladbach), PE-conjugated anti-human CD73 (#550257, lot 4149735, BD-Pharmingen, Heidelberg), FITC-CD90 (#11-0909-42, clone eBio5E10, lot 2648893, eBioscience, Frankfurt), PE-conjugated anti-human CD105 (#323206, clone 43A3, lot B418585, BioLegend, San Diego), PerCP-Cy5.5-conjugated anti-human CD14 (#45-0149-42, clone 61D3, lot 2785933, eBioscience), APC-conjugated anti-human CD31 (#17-0319-42, clone WM-59, lot 2702043, eBioscience), and FITC-conjugated anti-human CD34 (#343504, clone 581, lot B407007, eBioscience). ..

    Article Title: In-depth analysis of obesity-associated changes in adipose tissue-derived mesenchymal stromal/stem cells and primary cilia function.
    Article Snippet: Cells were trypsinized with 0.25% trypsin (Sigma-Aldrich), harvested, and fixed for 15min with icecold 2% paraformaldehyde. .. After washing twice with PBS, the cells were stained with the following antibodies: PE-conjugated anti-human cluster of differentiation 44 (CD44) (#130-113-342, clone REA690, lot 5240507442, MACS Miltenyi Biotec, Bergisch Gladbach), PE-conjugated anti-human CD73 (#550257, lot 4149735, BD-Pharmingen, Heidelberg), FITC-CD90 (#11-0909-42, clone eBio5E10, lot 2648893, eBioscience, Frankfurt), PEconjugated anti-human CD105 (#323206, clone 43A3, lot B418585, BioLegend, San Diego), PerCP-Cy5.5-conjugated anti-human CD14 (#45- 0149-42, clone 61D3, lot 2785933, eBioscience), APC-conjugated antihuman CD31 (#17-0319-42, clone WM-59, lot 2702043, eBioscience), and FITC-conjugated anti-human CD34 (#343504, clone 581, lot B407007, eBioscience). ..



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    Image Search Results


    Network-based hub gene screening and expression validation in OSCC. (A) Top 10 hub candidates ranked by betweenness centrality. (B) Top 10 hub candidates ranked by degree centrality. (C) Intersection of two rankings identifying CCNA2, CD44, and STAT1 as shared hub genes. (D–F) Expression of CCNA2, CD44, and STAT1 in GSE30784 . (G–I) Expression of CCNA2, CD44, and STAT1 in GSE9844 .

    Journal: Frontiers in Molecular Biosciences

    Article Title: Integrated network analysis and experimental validation identify CCNA2, CD44, and STAT1 as clinically relevant hub genes in oral squamous cell carcinoma

    doi: 10.3389/fmolb.2026.1748821

    Figure Lengend Snippet: Network-based hub gene screening and expression validation in OSCC. (A) Top 10 hub candidates ranked by betweenness centrality. (B) Top 10 hub candidates ranked by degree centrality. (C) Intersection of two rankings identifying CCNA2, CD44, and STAT1 as shared hub genes. (D–F) Expression of CCNA2, CD44, and STAT1 in GSE30784 . (G–I) Expression of CCNA2, CD44, and STAT1 in GSE9844 .

    Article Snippet: Primary antibodies against Cyclin A2 (CCNA2) (Abcam, ab181973), Cluster of Differentiation 44 (CD44) (Cell Signaling Technology, #3570), and Signal Transducer and Activator of Transcription 1 (STAT1) (Proteintech, 10144-2-AP) were applied, followed by HRP-conjugated secondary antibodies and DAB detection with hematoxylin counterstaining.

    Techniques: Expressing, Biomarker Discovery

    Validation and characterization of hub genes in TCGA cohort. (A–C) mRNA expression of CCNA2, CD44 and STAT1 in TCGA OSCC vs. normal tissues. (D–F) Kaplan–Meier overall survival curves stratified by CCNA2, CD44 and STAT1 expression. (G–I) GSEA enrichment plots for CCNA2, CD44 and STAT1.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Integrated network analysis and experimental validation identify CCNA2, CD44, and STAT1 as clinically relevant hub genes in oral squamous cell carcinoma

    doi: 10.3389/fmolb.2026.1748821

    Figure Lengend Snippet: Validation and characterization of hub genes in TCGA cohort. (A–C) mRNA expression of CCNA2, CD44 and STAT1 in TCGA OSCC vs. normal tissues. (D–F) Kaplan–Meier overall survival curves stratified by CCNA2, CD44 and STAT1 expression. (G–I) GSEA enrichment plots for CCNA2, CD44 and STAT1.

    Article Snippet: Primary antibodies against Cyclin A2 (CCNA2) (Abcam, ab181973), Cluster of Differentiation 44 (CD44) (Cell Signaling Technology, #3570), and Signal Transducer and Activator of Transcription 1 (STAT1) (Proteintech, 10144-2-AP) were applied, followed by HRP-conjugated secondary antibodies and DAB detection with hematoxylin counterstaining.

    Techniques: Biomarker Discovery, Expressing

    Prognostic nomogram and diagnostic performance of CCNA2, CD44 and STAT1. (A) Nomogram model based on CCNA2, CD44 and STAT1. (B) Calibration curve of the nomogram for 1-year survival prediction. (C) ROC curves of CCNA2, CD44 and STAT1 in GSE30784 . (D) ROC curves of CCNA2, CD44 and STAT1 in GSE9844 . (E) ROC curves of CCNA2, CD44 and STAT1 in TCGA.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Integrated network analysis and experimental validation identify CCNA2, CD44, and STAT1 as clinically relevant hub genes in oral squamous cell carcinoma

    doi: 10.3389/fmolb.2026.1748821

    Figure Lengend Snippet: Prognostic nomogram and diagnostic performance of CCNA2, CD44 and STAT1. (A) Nomogram model based on CCNA2, CD44 and STAT1. (B) Calibration curve of the nomogram for 1-year survival prediction. (C) ROC curves of CCNA2, CD44 and STAT1 in GSE30784 . (D) ROC curves of CCNA2, CD44 and STAT1 in GSE9844 . (E) ROC curves of CCNA2, CD44 and STAT1 in TCGA.

    Article Snippet: Primary antibodies against Cyclin A2 (CCNA2) (Abcam, ab181973), Cluster of Differentiation 44 (CD44) (Cell Signaling Technology, #3570), and Signal Transducer and Activator of Transcription 1 (STAT1) (Proteintech, 10144-2-AP) were applied, followed by HRP-conjugated secondary antibodies and DAB detection with hematoxylin counterstaining.

    Techniques: Diagnostic Assay

    Experimental validation of CCNA2, CD44 and STAT1 expression in OSCC tissues and cell lines. (A) Representative IHC staining of CCNA2, CD44 and STAT1 in control and OSCC tissues. (B–D) Quantification of IHC staining index for CCNA2, CD44 and STAT1 in human oral tissues. (E–G) qPCR analysis of CCNA2, CD44 and STAT1 mRNA levels in human oral tissues. (H–J) qPCR analysis of CCNA2, CD44 and STAT1 mRNA levels in normal versus OSCC cell lines. (K,L) qPCR analysis of CRNN mRNA expression in human oral tissues and cell lines. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Integrated network analysis and experimental validation identify CCNA2, CD44, and STAT1 as clinically relevant hub genes in oral squamous cell carcinoma

    doi: 10.3389/fmolb.2026.1748821

    Figure Lengend Snippet: Experimental validation of CCNA2, CD44 and STAT1 expression in OSCC tissues and cell lines. (A) Representative IHC staining of CCNA2, CD44 and STAT1 in control and OSCC tissues. (B–D) Quantification of IHC staining index for CCNA2, CD44 and STAT1 in human oral tissues. (E–G) qPCR analysis of CCNA2, CD44 and STAT1 mRNA levels in human oral tissues. (H–J) qPCR analysis of CCNA2, CD44 and STAT1 mRNA levels in normal versus OSCC cell lines. (K,L) qPCR analysis of CRNN mRNA expression in human oral tissues and cell lines. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Primary antibodies against Cyclin A2 (CCNA2) (Abcam, ab181973), Cluster of Differentiation 44 (CD44) (Cell Signaling Technology, #3570), and Signal Transducer and Activator of Transcription 1 (STAT1) (Proteintech, 10144-2-AP) were applied, followed by HRP-conjugated secondary antibodies and DAB detection with hematoxylin counterstaining.

    Techniques: Biomarker Discovery, Expressing, Immunohistochemistry, Control